Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Biosynthesis of Nucleic Acids

Biosynthesis of Nucleic Acids

Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Involvement of NtInvVR1 in the increase of reducing sugars during the curing of virginia tobacco.

Scientific reports·2026

Same author

Correction: Nicotine-mediated effects in neuronal and mouse models of synucleinopathy.

Frontiers in neuroscience·2025

Same author

Author Correction: The clove (Syzygium aromaticum) genome provides insights into the eugenol biosynthesis pathway.

Communications biology·2025

Same author

Non-targeted analytical comparison of a heated tobacco product aerosol against mainstream cigarette smoke: does heating tobacco produce an inherently different set of aerosol constituents?

Analytical and bioanalytical chemistry·2024

Same author

Nicotine-mediated effects in neuronal and mouse models of synucleinopathy.

Frontiers in neuroscience·2023

Same author

Suppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.).

BMC genomics·2023

Search research articles

Related Experiment Video

Updated: Jan 4, 2026

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

Published on: January 31, 2014

Asparagine Synthesis During Tobacco Leaf Curing.

Lucien Bovet¹, Cecilia Cheval¹, Aurore Hilfiker¹

¹Philip Morris International R&D, Philip Morris Products SA, CH-2000 Neuchâtel, Switzerland.

Plants (Basel, Switzerland)

|November 14, 2019

Summary

Leaf curing significantly alters amino acid levels during senescence. The asparagine synthetase genes ASN1 and ASN5 are key regulators of asparagine metabolism in tobacco leaves.

Keywords:

asparagine asparagine synthetases curing nitrogen assimilation senescence tobacco

More Related Videos

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations

Published on: January 10, 2015

Related Experiment Videos

Last Updated: Jan 4, 2026

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

Published on: January 31, 2014

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations

Published on: January 10, 2015

Area of Science:

Plant Biology
Molecular Biology
Biochemistry

Background:

Leaf senescence is a genetically regulated process impacting plant chemistry.
Asparagine synthetases are crucial for nitrogen metabolism but their roles in senescence are unclear.

Purpose of the Study:

To investigate the impact of leaf curing on asparagine metabolism in tobacco.
To identify key asparagine synthetase genes involved in this process.

Main Methods:

Analysis of amino acid concentrations during leaf curing.
Quantitative analysis of asparagine synthetase gene expression.
RNA interference (RNAi) to study gene function in tobacco plants.

Main Results:

Leaf curing significantly altered concentrations of asparagine, glutamine, aspartate, and glutamate.
Expression of ASN1 and ASN5 genes was upregulated during curing.
ASN1-RNAi and ASN5-RNAi plants showed altered amino acid accumulation compared to wild-type.

Conclusions:

ASN1 and ASN5 are key regulators of asparagine metabolism during tobacco leaf curing.
Leaf curing differentially affects asparagine metabolism depending on the curing method (leaf vs. stalk).