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Tissue Homogenization and Cell Lysis

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Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
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Updated: Jan 3, 2026

Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells

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Published on: July 22, 2015

Cell lysis via acoustically oscillating sharp edges.

Zeyu Wang¹, Po-Hsun Huang¹, Chuyi Chen¹

¹Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA. phhuang73@gmail.com tony.huang@duke.edu.

|November 14, 2019

Summary

This summary is machine-generated.

This study presents a novel acoustofluidic device for efficient cell lysis. The device uses acoustic streaming to rupture cell membranes, achieving over 90% lysis efficiency without reagents, ideal for point-of-care applications.

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Area of Science:

Biomedical Engineering
Acoustofluidics
Cell Biology

Background:

Cell lysis is a critical step in many biological and biomedical analyses.
Existing cell lysis methods often require reagents and can be time-consuming or inefficient.
There is a need for rapid, reagent-free, and efficient cell lysis techniques.

Purpose of the Study:

To demonstrate an acoustofluidic device for reagent-free cell lysis.
To investigate the mechanism of cell membrane rupture via acoustic streaming-induced shear forces.
To evaluate the device's efficiency and applicability to various cell types and clinical samples.

Main Methods:

Fabrication of an acoustofluidic device with sharp-edged structures.
Induction of acoustic streaming using oscillating structures.
Application of acoustic streaming-derived shear forces for cell membrane rupture.
Continuous flow cell lysis experiments at various flow rates.

Main Results:

Achieved over 90% cell lysis efficiency across a range of sample flow rates.
Demonstrated successful lysis of both adherent and non-adherent cells.
Validated the device with clinically relevant samples, including malaria-infected red blood cells.
Enabled downstream protein and gene analysis without post-lysis washing steps.

Conclusions:

The acoustofluidic device provides an efficient, continuous, and reagent-free method for cell lysis.
The device is versatile, applicable to diverse cell types and clinical samples.
Its simplicity, low sample volume consumption, and suitability for downstream analysis make it valuable for point-of-care applications.