Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polymorphism of <i>pfmdr1</i> Gene Mutation Conferring Resistance to Artemisinin-Based Combination Therapy in <i>Plasmodium falciparum</i> in Patients at Gulu Regional Referral Hospital in Northern Uganda.

Journal of parasitology research·2026
Same author

Praziquantel efficacy and post-treatment reinfection and incidence of Schistosoma mansoni among school-aged children in Northern Uganda: a 24-week cohort study in a high transmission focus.

BMC infectious diseases·2026
Same author

Rapid colorimetric detection of Citrus tristeza virus combining portable sample preparation and reverse transcription-loop mediated isothermal amplification.

SLAS technology·2026
Same author

Prevalence, intensity, and risk factors of schistosomiasis and intestinal parasitic infections among primary school children in northern Uganda: Implications for public health interventions.

PLoS neglected tropical diseases·2025
Same author

Wicking in Paper-Based Devices with Engineered Surface Grooves.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Endosymbiont hijacking of acylcarnitines regulates insect vector fecundity by suppressing the viability of stored sperm.

PLoS genetics·2025
Same journal

AI/ML-Enabled Multi-Omics Integration of Host Genetics, Immunity, and the Gut Microbiome in Crohn's Disease: From Diagnosis to Theranostics.

SLAS technology·2026
Same journal

Spatial transcriptome and single-cell reveal the role of sorbitol metabolism in hepatocellular carcinoma progression and tumor microenvironment.

SLAS technology·2026
Same journal

An Adaptable Microplate-Integrated Purification Workflow Tailored for High-Throughput Automated Parallel Amide Syntheses.

SLAS technology·2026
Same journal

Revolutionizing Transcriptomics: From Single-Cell Insights to RNA-based Interventions.

SLAS technology·2026
Same journal

Smartphone-based colorimetric glucose biosensor using peroxidase-like activity of bimetallic catalyst supported onto graphitic carbon nitride nanosheets.

SLAS technology·2026
Same journal

XVCF: Exquisite visualization of VCF data from genomic experiments.

SLAS technology·2026
See all related articles

Related Experiment Video

Updated: Jan 3, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

9.0K

Distance and Microsphere Aggregation-Based DNA Detection in a Paper-Based Microfluidic Device.

Brent Kalish1, Jianhou Zhang1, Hilary Edema2

  • 1Department of Mechanical Engineering, University of California Riverside, Riverside, CA, USA.

SLAS Technology
|November 15, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a simple, quantitative DNA detection method using paper-based microfluidics. Polystyrene microsphere aggregation on filter paper correlates DNA concentration with wicking distance, enabling easy measurement without extra equipment.

Keywords:
DNA detectionaggregationdistance-basedmicrospherepaper-based

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.7K
Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.1K

Related Experiment Videos

Last Updated: Jan 3, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

9.0K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.7K
Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.1K

Area of Science:

  • Paper-based microfluidics
  • Quantitative biosensing
  • Nanomaterial-based diagnostics

Background:

  • Traditional paper-based microfluidic devices often yield only qualitative results.
  • Quantitative analysis in microfluidics typically requires complex instrumentation.
  • Distance-based readout methods offer a user-friendly alternative for quantitative data acquisition.

Purpose of the Study:

  • To develop a quantitative DNA detection device using paper-based microfluidics.
  • To leverage polystyrene microsphere aggregation for DNA concentration readout.
  • To establish a correlation between DNA target concentration and microsphere wicking distance.

Main Methods:

  • Conjugation of polystyrene microspheres with single-stranded DNA (ssDNA) oligomers.
  • Utilizing ssDNA hybridization to induce microsphere aggregation in the presence of target DNA.
  • Measuring the wicking distance of aggregated microspheres through filter paper as a quantitative indicator.

Main Results:

  • Microsphere aggregation was observed, influenced by target DNA concentration and incubation time.
  • Increased target DNA concentration led to larger aggregates and reduced microsphere wicking distance.
  • The developed device successfully detected target DNA spiked in extracted plant DNA samples.

Conclusions:

  • A novel quantitative DNA detection method based on distance-based readout in paper microfluidics was developed.
  • Polystyrene microsphere aggregation provides a reliable mechanism for visual, quantitative DNA detection.
  • This approach offers a simple, equipment-free solution for DNA quantification, applicable to biological samples.