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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Published on: April 8, 2020
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A hyaluronidase/ATP tandem stimuli-responsive supramolecular assembly
Jie Gao1, Huijuan Yu, Fang-Yuan Chen
1College of Chemistry, Key Laboratory of Functional Polymer Materials (Ministry of Education), State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, 300071, P. R. China. dshguo@nankai.edu.cn.
Chemical Communications (Cambridge, England)
|November 15, 2019
Summary
Researchers developed a novel drug delivery system using modified calixarene and hyaluronic acid. This system precisely releases therapeutic agents in response to tumor biomarkers like hyaluronidase and adenosine triphosphate (ATP), enabling targeted phototheranostics.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery Systems
Background:
- Developing targeted drug delivery systems is crucial for improving therapeutic efficacy and reducing side effects.
- Tumor microenvironments often exhibit unique biochemical markers that can be exploited for targeted therapies.
- Hyaluronic acid (HA) is a polymer that can be functionalized for drug delivery and is responsive to specific enzymes.
Purpose of the Study:
- To design and characterize a novel tandem stimuli-responsive assembly for targeted drug delivery.
- To investigate the triggered release of eosin Y (EY) from the assembly in response to tumor-specific biomarkers.
- To evaluate the potential of this system for tumor phototheranostics.
Main Methods:
- Synthesis of guanidinium-modified calix[5]arene (GC5A-6C) and eosin Y modified hyaluronic acid (EY-HA).
- Construction of a tandem stimuli-responsive assembly by combining GC5A-6C and EY-HA.
- Investigation of the assembly's disassembly triggered by hyaluronidase.
- Analysis of adenosine triphosphate (ATP)-activated release of EY from the assembly.
Main Results:
- The designed GC5A-6C and EY-HA assembly demonstrated stimuli-responsive behavior.
- Hyaluronidase successfully triggered the disassembly of the assembly.
- Adenosine triphosphate (ATP) effectively activated the release of eosin Y (EY).
- Both hyaluronidase and ATP are recognized tumor biomarkers, indicating specific targeting capability.
Conclusions:
- The developed tandem stimuli-responsive assembly shows precise control over drug release.
- The system's responsiveness to hyaluronidase and ATP highlights its potential for targeted cancer therapy.
- This platform holds promise for advanced tumor phototheranostics, enabling precise delivery and treatment.
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