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Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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Long non-coding RNA FENDRR restrains the aggressiveness of CRC via regulating miR-18a-5p/ING4 axis

Sheng Lu Yin1, Fei Xiao1, Yong Fu Liu1

  • 1The Department of the Emergency Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Journal of Cellular Biochemistry
|November 15, 2019
PubMed

Insights

Long non-coding RNA FENDRR is downregulated in colorectal cancer (CRC), suppressing tumor growth and metastasis. It functions by interacting with miR-18a-5p to regulate ING4 expression, offering potential therapeutic strategies for CRC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific involvement and clinical significance of FENDRR in colorectal cancer (CRC) remain largely unexplored.

Purpose of the Study:

  • To investigate the role and mechanism of lncRNA FENDRR in colorectal cancer (CRC).
  • To determine the correlation between FENDRR expression and clinical outcomes in CRC patients.

Main Methods:

  • Quantitative real-time PCR to assess FENDRR and ING4 expression.
  • In vitro cell proliferation, migration, and invasion assays.
  • In vivo tumor growth and metastasis models.
  • RNA immunoprecipitation and dual-luciferase reporter assays to confirm molecular interactions.

Main Results:

  • FENDRR expression is significantly downregulated in CRC tissues and inversely correlated with advanced stage and poor prognosis.
  • Overexpression of FENDRR inhibits CRC cell proliferation, migration, and invasion in vitro, and suppresses tumor growth and metastasis in vivo.
  • FENDRR directly interacts with miR-18a-5p, leading to the downregulation of ING4 expression. Restoration of ING4 or inhibition of miR-18a-5p can rescue FENDRR's tumor-suppressive effects.

Conclusions:

  • FENDRR acts as a tumor suppressor in colorectal cancer.
  • The FENDRR/miR-18a-5p/ING4 axis plays a critical role in regulating CRC progression.
  • FENDRR may serve as a potential diagnostic biomarker and therapeutic target for colorectal cancer.
Keywords:
CRCFEMDRRING4miR-18a-5p

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