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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

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Multiplexed Immunosensors and Immunoarrays

Abby Jones¹, Lasangi Dhanapala¹, Rumasha N T Kankanamage¹

¹Department of Chemistry , University of Connecticut , 55 North Eagleville Road , Storrs , Connecticut 06269 , United States.

Analytical Chemistry

|November 16, 2019

Summary

No abstract available in PubMed .

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