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Quantification of eIF2alpha phosphorylation during immunogenic cell death.

Lucillia Bezu¹, Alejandra Wu Chuang², Juliette Humeau²

¹Service anesthésie-réanimation, Hôpital Européen Georges Pompidou, AP-HP, Paris, France; Equipe labellisée Ligue Nationale Contre le Cancer, Université Paris Descartes, Université Sorbonne Paris Cité, Université Paris Diderot, Institut National de la Santé et de la Recherche Médicale (INSERM), UMR1138, Centre de Recherche des Cordeliers, Paris, France; Metabolomics and Cell Biology Platforms, Institut Gustave Roussy, Villejuif, France.

Methods in Enzymology

|November 16, 2019

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Summary

Immunogenic cell death (ICD) triggers an anticancer immune response. Detecting eIF2alpha phosphorylation is key to assessing ICD and its ability to release danger signals that enhance tumor immunity.

Area of Science:

Oncology
Immunology
Cell Biology

Background:

Immunogenic cell death (ICD) is a form of cell death induced by certain chemotherapy drugs.
ICD initiates an adaptive immune response against tumor cells by releasing danger signals.
These signals promote dendritic cell maturation and cytotoxic T lymphocyte priming.

Purpose of the Study:

To describe methods for detecting eIF2alpha phosphorylation.
To assess the role of eIF2alpha phosphorylation in ICD.

Main Methods:

Techniques for detecting eIF2alpha phosphorylation are detailed.
Assessment of ICD through eIF2alpha phosphorylation analysis.

Main Results:

Eukaryotic translation initiation factor 2-alpha (EIF2A) phosphorylation is a hallmark of ICD.

Keywords:

Cancer Endoplasmic reticulum stress Immune response Mitoxantrone Oxaliplatin

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This phosphorylation event regulates the release of damage-associated molecular patterns (DAMPs), such as ATP and calreticulin.

DAMPs are crucial for initiating the adaptive immune response against tumors.

Conclusions:

Detection of eIF2alpha phosphorylation provides a reliable method for assessing ICD.
Understanding EIF2A phosphorylation in ICD can lead to improved cancer immunotherapies.