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Genome-Scale Transcript Analyses of Human Neutrophils
Scott D Kobayashi1, Adeline R Porter1, Sarah L Anzick2
1Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 16, 2019
Summary
This study details standardized methods for analyzing human neutrophil gene expression using microarrays and next-generation sequencing. These techniques offer new insights into resolving infections by examining polymorphonuclear leukocyte transcript levels.
Area of Science:
- Molecular Biology
- Immunology
- Genomics
Background:
- Transcriptome analysis has transformed biological and pathological understanding.
- Polymorphonuclear leukocyte (PMN) gene expression studies offer insights into human infection resolution.
Purpose of the Study:
- To present standardized methods for analyzing human neutrophil gene expression.
- To enable evaluation of PMN transcript levels using accessible technologies.
Main Methods:
- Utilized Affymetrix oligonucleotide microarrays for gene expression analysis.
- Employed next-generation sequencing for comprehensive transcript profiling.
- Developed procedures using commercially available reagents and materials.
Main Results:
- Established standardized protocols for human neutrophil gene expression analysis.
- Demonstrated the utility of microarrays and next-generation sequencing for PMN transcript evaluation.
Conclusions:
- The presented methods provide a standardized approach for assessing human neutrophil gene expression.
- These techniques facilitate deeper understanding of molecular processes in infection resolution.
