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Updated: Jan 3, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

Quantitative Jasmonate Profiling Using a High-Throughput UPLC-NanoESI-MS/MS Method.

Cornelia Herrfurth^1,2, Ivo Feussner^3,4,5

¹Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Goettingen, Goettingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|November 18, 2019

Summary

This summary is machine-generated.

Quantifying plant hormones like jasmonates is challenging due to their numerous forms and low concentrations. This study introduces a sensitive liquid chromatography-mass spectrometry method for accurate jasmonate measurement in plants.

Keywords:

Deuterium-labeled standards Jasmonates Jasmonic acid Liquid chromatography Mass spectrometry Nanoelectrospray ionization Two-phase extraction

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

Area of Science:

Plant biology
Plant hormone signaling
Analytical chemistry

Background:

Jasmonates (JA) are crucial plant hormones regulating stress responses and development.
Measuring diverse jasmonate derivatives at low concentrations in plant tissues is analytically challenging.
Existing methods often lack the sensitivity and specificity required for comprehensive jasmonate profiling.

Purpose of the Study:

To develop and validate a selective and sensitive method for quantifying jasmonate levels in plant tissues and fluids.
To enable the absolute quantification of multiple jasmonate derivatives.
To facilitate semi-quantitative analysis of other related metabolites in the jasmonate pathway.

Main Methods:

A two-phase extraction technique was employed.
Liquid chromatography coupled with nanoelectrospray ionization and mass spectrometry (LC-nESI-MS) was utilized.
Stable deuterium-labeled standards and authentic standards were used for absolute and semi-quantitative analysis.

Main Results:

The developed method demonstrates high selectivity and sensitivity for jasmonate determination.
Absolute quantification of numerous jasmonate derivatives was achieved.
Semi-quantitative analysis of additional jasmonate pathway metabolites was also successful.

Conclusions:

This novel analytical method provides a robust tool for precise jasmonate profiling in various plant species.
The technique overcomes previous limitations in measuring these vital plant signaling compounds.
It will aid in advancing research on plant stress responses and developmental processes regulated by jasmonates.