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A dual-responsive biosensor for blood lead detection.

Huan Chen1, Shuibin Shao1, Yueqi Yu1

  • 1National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.

Analytica Chimica Acta
|November 19, 2019
PubMed
Summary
This summary is machine-generated.

A new dual-responsive biosensor using magnetic hyperbranched polyamide with heparin modification (MHPAM-H) offers simple and accurate detection of blood lead. This novel method provides sensitive electrochemical and fluorescent signals for heavy metal analysis.

Keywords:
Blood compatibilityBlood lead ionsDual-responsive biosensorElectrochemical/ fluorescent detection

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Materials Science

Background:

  • Accurate detection of trace heavy metals, particularly lead, in blood is crucial for public health.
  • Existing methods for blood lead detection can be complex and lack sensitivity.
  • Development of novel biosensors is needed for improved heavy metal analysis.

Purpose of the Study:

  • To develop a novel dual-responsive electrochemical/fluorescent biosensor for sensitive blood lead detection.
  • To investigate the properties and performance of the developed biosensor in both aqueous and blood samples.
  • To evaluate the potential application of the biosensor for future clinical diagnostics.

Main Methods:

  • Fabrication of a magnetic hyperbranched polyamide with heparin modification (MHPAM-H) based dual-responsive biosensor.
  • Conjugation of the biosensor with blood lead ions to induce electrochemical and fluorescent signals.
  • Characterization of blood biocompatibility, sensor construction, and detection performance.
  • Assessment of selectivity and stability of the biosensor for lead ion detection.

Main Results:

  • The MHPAM-H biosensor successfully exhibited both electrochemical and fluorescent signals upon binding with lead ions.
  • The biosensor demonstrated good linearity over a wide range (1.5 pM–4.8 × 10³ pM electrochemical; 0.5 pM–4.8 × 10³ pM fluorescent).
  • Achieved low detection limits of 4.4 pM (electrochemical) and 1.0 pM (fluorescent) for blood lead detection.

Conclusions:

  • The developed dual-responsive biosensor offers a simple, accurate, and sensitive method for blood lead detection.
  • The MHPAM-H based biosensor shows significant potential for practical application in diagnosing lead poisoning.
  • This novel biosensor advances heavy metal detection capabilities in complex biological matrices.