Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 3, 2026

Imaging Dpp Release from a Drosophila Wing Disc
06:12

Imaging Dpp Release from a Drosophila Wing Disc

Published on: October 30, 2019

6.1K

Imaging Dpp Release from a Drosophila Wing Disc.

Laura Faith George1, Emily Anne Bates2

  • 1Department of Pediatrics, University of Colorado School of Medicine.

Journal of Visualized Experiments : Jove
|November 19, 2019
PubMed
Summary

Researchers developed a new method to measure the release of Decapentaplegic (Dpp), a transforming Growth Factor-beta (TGF-β) superfamily ligand, from cells. This technique is crucial for understanding developmental signaling timing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SPEN deficiency contributes to the development of orofacial clefts in humans and mice.

Human molecular genetics·2026
Same author

Oral Consumption of Cannabidiol During Pregnancy Alters Behavior in Mouse Offspring.

Cannabis and cannabinoid research·2026
Same author

WNT4 deficiency impacts heart, diaphragm, and palate development: Insights from human genetics, machine learning, and mouse models.

Developmental biology·2025
Same author

Kcnj2 regulates electrical activity-induced gene networks in embryonic mouse palate shelves.

Developmental biology·2025
Same author

Consumption of clinically relevant cannabidiol doses during pregnancy alters postnatal behavior in offspring.

bioRxiv : the preprint server for biology·2025
Same author

Depolarization induces calcium-dependent BMP4 release from mouse embryonic palate mesenchymal cells.

Nature communications·2024

Area of Science:

  • Developmental Biology
  • Molecular Signaling
  • Genetics

Background:

  • The transforming Growth Factor-beta (TGF-β) superfamily plays a critical role in embryonic patterning and adult structure development.
  • Ligand concentration and timing of exposure are key factors influencing TGF-β superfamily signaling outcomes.
  • Understanding the release kinetics of these signaling molecules from producing cells is essential for deciphering developmental processes.

Purpose of the Study:

  • To establish a method for quantifying the release of Decapentaplegic (Dpp), a Drosophila BMP ligand, from specific cellular sources.
  • To enable the study of ligand release timing as a critical parameter in developmental signaling.

Main Methods:

  • Development of a novel assay to measure the release of the Drosophila BMP ligand, Decapentaplegic (Dpp).

More Related Videos

A GFP Complementation-based Dual-expression System for Assessing Cell-Cell Contact Mediated by Cytonemes in Live Drosophila Wing Imaginal Discs
07:26

A GFP Complementation-based Dual-expression System for Assessing Cell-Cell Contact Mediated by Cytonemes in Live Drosophila Wing Imaginal Discs

Published on: August 22, 2025

566
Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

27.5K

Related Experiment Videos

Last Updated: Jan 3, 2026

Imaging Dpp Release from a Drosophila Wing Disc
06:12

Imaging Dpp Release from a Drosophila Wing Disc

Published on: October 30, 2019

6.1K
A GFP Complementation-based Dual-expression System for Assessing Cell-Cell Contact Mediated by Cytonemes in Live Drosophila Wing Imaginal Discs
07:26

A GFP Complementation-based Dual-expression System for Assessing Cell-Cell Contact Mediated by Cytonemes in Live Drosophila Wing Imaginal Discs

Published on: August 22, 2025

566
Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

27.5K
  • Application of the method to cells within the wing primordium or wing disc of Drosophila.

    • Main Results:

    • Successfully demonstrated a method to measure the release of Dpp from Drosophila wing disc cells.
    • Provides a tool to investigate the temporal dynamics of ligand secretion in developmental contexts.

    Conclusions:

    • The timing of ligand release is as important as concentration for developmental signaling.
    • The developed method offers a versatile approach applicable to other signaling ligands and biological systems.
    • This research advances the understanding of how developmental signals are regulated spatially and temporally.