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Updated: Jan 3, 2026
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Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
Published on: June 14, 2020
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Histological and Histochemical Observations on Mast Cell and Glands in Chronic Tonsillitis
Sushil Gaur1, O N Sinha1, Ashesh Bhushan1
1C307 Black Gold Apartment, Omega 1, Greater Noida, Up 201308 India.
Summary
This study investigated mast cells and heparin in chronic tonsillitis. Mast cells were found concentrated in tonsil capsules, with their heparin content varying, suggesting a role in allergic inflammation.
Area of Science:
- Immunology
- Histopathology
Background:
- Mast cells are key immune cells involved in hypersensitivity and allergic reactions.
- Allergic reactions frequently affect the upper aero-digestive tract, including the pharynx and respiratory system.
Purpose of the Study:
- To investigate the distribution and histochemical characteristics of mast cells in human palatine tonsils of patients with chronic tonsillitis.
- To explore the role of different types of heparin within mast cells in the context of tonsillitis.
Main Methods:
- Histological and histochemical analysis of 20 human palatine tonsil samples from patients with chronic tonsillitis.
- Mast cell morphology and distribution were assessed using Harris Hematoxylin and Eosin staining.
- Metachromatic reactions with toluidine blue at pH 2 and 4.4 were used to identify heparin sulfates in mast cell granules.
Main Results:
- Mast cells were observed in all parts of the tonsillar tissue, with higher concentrations in the capsule (4-5/hpf) compared to the lamina propria and connective tissue septa (1-2/hpf).
- Mast cells exhibited varied morphology, including oval, elongated, fusiform, and pleomorphic shapes.
- Histochemical analysis indicated the presence of heparin in mast cell granules, with varying sulfate content.
Conclusions:
- Mast cells are present in chronic tonsillitis, with a notable concentration in the tonsillar capsule.
- The histochemical properties of mast cell granules suggest a role for heparin in the inflammatory processes of chronic tonsillitis.
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