Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells
1The Szalmuk Family Department of Medical Oncology, Level 2, Cabrini Institute, Malvern, VIC, Australia. TLang@cabrini.com.au.
Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2019
Abstract:
Macrophage migration inhibitory factor (MIF) is expressed and released ubiquitously by numerous cell types and tissues. MIF is detected and constitutively expressed at the protein level both intra- and extracellularly. This chapter outlines methods for cultivating, purifying, detecting, and quantifying concentrations of MIF from murine primary derived macrophages and dendritic cell culture supernatants.
