Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Energetic coupling between DNA bending and base pair opening.

J Ramstein1, R Lavery

  • 1Centre de Biophysique Moléculaire, Orléans, France.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Age adjusted D-dimer in the Belfast Health and Social Care Trust: A retrospective study.

The Ulster medical journal·2018
Same author

Improving the treatment of coarse-grain electrostatics: CVCEL.

The Journal of chemical physics·2016
Same author

Energetic and conformational aspects of A:T base-pair opening within the DNA double helix.

Chemphyschem : a European journal of chemical physics and physical chemistry·2013
Same author

A Mechanism for RecA-Promoted Sequence Homology Recognition and Strand Exchange Between Single-Stranded DNA and Duplex DNA, via Triple-Helical Intermediates.

Journal of biomolecular structure & dynamics·2012
Same author

Conformational analysis of nucleic acids revisited: Curves+.

Nucleic acids research·2009
Same author

Genetics of ovarian differentiation: Rspo1, a major player.

Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation·2008
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Investigating base pair opening in DNA, this study reveals that DNA bending significantly aids this process. Conversely, disrupted base pairs promote DNA bending, suggesting a coupled mechanism crucial for biological reactions.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Computational Chemistry

Background:

  • Understanding DNA dynamics is crucial for various biological processes.
  • Base pair opening is a key step in DNA replication and transcription.
  • Previous studies relied on indirect measurements like hydrogen exchange.

Purpose of the Study:

  • To investigate the energetic pathway of base pair opening in B-DNA.
  • To explore the interplay between DNA base pair opening and DNA bending.
  • To elucidate the role of energetic coupling in nucleic acid distortion.

Main Methods:

  • Theoretical molecular modeling was employed to simulate DNA behavior.
  • Simulations focused on the disruption of individual base pairs within a B-DNA duplex.

Related Experiment Videos

  • Energetic profiles of base pair opening and DNA bending were analyzed.

    • Main Results:

    • The energetic cost of single base pair disruption aligns with hydrogen exchange data.
    • DNA bending was found to significantly facilitate the base pair opening process.
    • Disruption of a base pair was observed to enhance DNA's propensity to bend.

    Conclusions:

    • Energetic coupling between base pair opening and DNA bending is proposed.
    • This coupled mechanism likely plays a significant role in biological reactions involving DNA distortion.
    • Theoretical modeling provides valuable insights into DNA structural dynamics.