Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

2.6K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.6K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

3.4K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.4K
Destabilization of Microtubules01:45

Destabilization of Microtubules

3.4K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
3.4K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.1K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.1K
Drugs that Destabilize Microtubules01:10

Drugs that Destabilize Microtubules

3.6K
Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scaling Up Task-Shared Depression Care in Texas: Evaluating the Preliminary Effectiveness and Acceptability of a Digital Program for Training Non-Specialist Providers.

Psychiatric research and clinical practice·2026
Same author

Site Confidence Scores among All Available Structures to Help Infer Conformational Origins of Identified Cross-Links.

Journal of proteome research·2026
Same author

Neoadjuvant chemotherapy with bevacizumab for locally advanced vulvar cancer.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2025
Same author

HSR25-192: Evaluation of Alternative Approaches to New Lesion Management and Measurable Disease Size Threshold on RANO-BM Response Assessment Following SRS for Brain Metastases.

Journal of the National Comprehensive Cancer Network : JNCCN·2025
Same author

Large-Scale Quantitative Cross-Linking and Mass Spectrometry Provide New Insight into Protein Conformational Plasticity within Organelles, Cells, and Tissues.

Journal of proteome research·2025
Same author

Large-Scale Quantitative Cross-Linking and Mass Spectrometry Provides New Insight on Protein Conformational Plasticity within Organelles, Cells, and Tissues.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: Jan 3, 2026

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
08:29

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

Published on: May 14, 2018

10.4K

Cellular Interactome Dynamics during Paclitaxel Treatment.

Juan D Chavez1, Andrew Keller1, Bo Zhou2

  • 1Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA.

Cell Reports
|November 21, 2019
PubMed
Summary

Paclitaxel cancer therapy affects more than just microtubules. This study reveals how paclitaxel alters protein structures and interactions, impacting cell homeostasis and apoptosis signaling.

Keywords:
ATP synthasechemical cross-linkinginteractomemass spectrometrymicrotubulesmitochondriamitotic inhibitorpaclitaxelquantitative analysisstable isotope labeling

More Related Videos

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers
06:02

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers

Published on: February 20, 2017

7.9K
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.4K

Related Experiment Videos

Last Updated: Jan 3, 2026

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
08:29

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

Published on: May 14, 2018

10.4K
Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers
06:02

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers

Published on: February 20, 2017

7.9K
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.4K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Cancer Research

Background:

  • Paclitaxel is a widely used cancer therapy that inhibits cell-cycle progression.
  • Its primary mechanism involves stabilizing microtubules, but this doesn't fully explain observed effects.
  • Drug resistance and toxic side effects limit paclitaxel's efficacy.

Purpose of the Study:

  • To investigate the systems-level changes in protein structure and interactions induced by paclitaxel.
  • To identify novel targets and mechanisms beyond microtubule stabilization.

Main Methods:

  • Quantitative chemical cross-linking with mass spectrometry (CX-MS) was applied to paclitaxel-treated cells.
  • CX-MS enabled large-scale measurements of protein levels, conformations, and interactions.

Main Results:

  • Paclitaxel treatment induced drug concentration-dependent changes in protein levels and conformations.
  • Alterations were observed in tubulins, apoptotic signaling proteins, and proteins involved in cellular homeostasis.
  • Novel protein interactions and structural changes were identified.

Conclusions:

  • Paclitaxel exerts its effects through a broader range of molecular targets and pathways than previously understood.
  • Understanding these systems-level changes can inform strategies to overcome drug resistance and reduce toxicity.
  • This study provides a foundation for exploring paclitaxel's multifaceted impact on cellular processes.