Competition Between Chemolithotrophic Acetogenesis and Hydrogenotrophic Methanogenesis for Exogenous H₂/CO₂ in...

Anaerobic digestion is a widely applied technology for sewage sludge treatment. Hydrogen and CO₂ are important degradation products, which serve as substrates for both hydrogenotrophic methanogenesis and chemolithotrophic acetogenesis. In order to understand the competition between these processes for H₂/CO₂, sludge samples were incubated under H₂/CO₂ headspace at different temperatures, and analyzed with respect to turnover of H₂, CO₂, CH₄ and acetate including their δ¹³C values. At 15°C, ¹³C-depleted acetate (δ¹³C of -41 to -43‰) and transient acetate accumulation were observed under H₂/CO₂, and CH₄ accumulated with δ¹³C values increasing from -53 to -33‰. The copy numbers of the fhs gene, which is characteristic for acetogenic bacteria, were at 15°C one order of magnitude higher in the H₂/CO₂ incubations than the N₂ control. At 30°C, however, acetate did not accumulate in the H₂/CO₂ incubation and the δ¹³C of CH₄ was very low (-100 to -77‰). At 50°C, isotopically enriched acetate was transiently formed and subsequently consumed followed by the production of ¹³C-depleted CH₄. Collectively, the results indicate a high contribution of chemolithotrophic acetogenesis to H₂/CO₂ utilization at 15°C and 50°C, while H₂/CO₂ was mainly consumed by hydrogenotrophic methanogenesis at 30°C. Fermentative production and methanogenic consumption of acetate were active at 50°C.