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Coding-Complete Genome Sequence of Staphylococcus aureus Podophage Portland

Rachele M Bonasera1, Abby Korn1, Heather Newkirk1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|November 23, 2019
PubMed

Insights

We sequenced the genome of Staphylococcus aureus podophage Portland, a virus closely related to other phi29-like phages. This provides new data on these important bacterial viruses.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • *Staphylococcus aureus* is a significant pathogen causing diverse infections, particularly multidrug-resistant strains.
  • *S. aureus* infections pose a substantial public health challenge.
  • Bacteriophages (phages) are viruses that infect bacteria and are being explored for therapeutic applications.

Purpose of the Study:

  • * To announce the genome sequence of *S. aureus* podophage Portland.
  • * To characterize its relationship to other known phi29-like *S. aureus* phages.

Main Methods:

  • * Genome sequencing of phage Portland.
  • * Bioinformatic analysis and comparison with related phage genomes.

Main Results:

  • * The genome sequence of *S. aureus* podophage Portland was determined.
  • * Phage Portland was identified as closely related to phi29-like *S. aureus* podophages, including phi44AHJD and phiP68.
  • * The precise genome termini of phage Portland could not be resolved due to potential terminal protein obscuration.

Conclusions:

  • * The genome sequence of phage Portland adds to the genomic resources for *S. aureus* phages.
  • * Understanding the genomics of *S. aureus* phages is crucial for developing phage-based therapies against resistant bacteria.

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