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Coding-Complete Genome Sequence of Staphylococcus aureus Podophage Portland
Rachele M Bonasera1, Abby Korn1, Heather Newkirk1
1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
Microbiology Resource Announcements
|November 23, 2019
Summary
We sequenced the genome of Staphylococcus aureus podophage Portland, a virus closely related to other phi29-like phages. This provides new data on these important bacterial viruses.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- *Staphylococcus aureus* is a significant pathogen causing diverse infections, particularly multidrug-resistant strains.
- *S. aureus* infections pose a substantial public health challenge.
- Bacteriophages (phages) are viruses that infect bacteria and are being explored for therapeutic applications.
Purpose of the Study:
- * To announce the genome sequence of *S. aureus* podophage Portland.
- * To characterize its relationship to other known phi29-like *S. aureus* phages.
Main Methods:
- * Genome sequencing of phage Portland.
- * Bioinformatic analysis and comparison with related phage genomes.
Main Results:
- * The genome sequence of *S. aureus* podophage Portland was determined.
- * Phage Portland was identified as closely related to phi29-like *S. aureus* podophages, including phi44AHJD and phiP68.
- * The precise genome termini of phage Portland could not be resolved due to potential terminal protein obscuration.
Conclusions:
- * The genome sequence of phage Portland adds to the genomic resources for *S. aureus* phages.
- * Understanding the genomics of *S. aureus* phages is crucial for developing phage-based therapies against resistant bacteria.
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