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Nucleocapsid proteins from other swine enteric coronaviruses differentially modulate PEDV replication
Suttipun Sungsuwan1, Anan Jongkaewwattana1, Peera Jaru-Ampornpan1
1Virology and Cell Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand.
Virology
|November 23, 2019
Summary
Porcine epidemic diarrhea virus (PEDV) replication is affected by other swine coronaviruses. Porcine deltacoronavirus (PDCoV) N protein suppresses PEDV, while transmissible gastroenteritis virus (TGEV) N protein enhances it.
Area of Science:
- Veterinary Virology
- Molecular Virology
- Infectious Disease
Background:
- Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine deltacoronavirus (PDCoV) infect swine intestinal cells.
- The impact of co-infection with these viruses on viral replication and pathogenicity is largely unknown.
Purpose of the Study:
- To investigate the interactions between different coronavirus nucleocapsid (CoV N) proteins.
- To determine how these interactions affect PEDV replication during co-infection.
Main Methods:
- Investigated nucleocapsid protein interactions using co-infection models.
- Assessed the impact of PDCoV N and TGEV N on PEDV replication, RNA synthesis, and virion production.
Main Results:
- PDCoV N and TGEV N competitively interact with PEDV N.
- PDCoV N significantly suppresses PEDV replication.
- TGEV N enhances PEDV RNA and virion production, similar to PEDV N.
- Endogenous PEDV N is essential for producing infectious PEDV particles, despite partial protein interchangeability.
Conclusions:
- Coronavirus nucleocapsid proteins exhibit functional compatibilities and incompatibilities.
- These interactions offer insights into viral co-infection dynamics and coronavirus evolution.
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