Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Transformation01:33

Bacterial Transformation

59.2K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
59.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunocyte senescence: A new perspective on the remodeling of the ovarian cancer microenvironment and therapeutic intervention.

Journal of pharmaceutical analysis·2026
Same author

Early-life mitochondrial impact of prenatal DL-PCB exposure: A signal from cord blood mtDNA.

Ecotoxicology and environmental safety·2026
Same author

Leveraging the microscale effect to enhance the overload capacity of a piezoresistive differential pressure sensor.

Microsystems & nanoengineering·2026
Same author

d-amino acids restrain macrophage IL-1β release through gasdermin D acetylation.

Science advances·2026
Same author

Synergistic Effects of Nannochloropsis oculata Enzymatic Hydrolysates and Soybean Lecithin in Enhancing the Delivery Efficiency of Lutein in Grape Seed Oil Emulsions.

Journal of food science·2026
Same author

Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.

Molecular pharmaceutics·2026

Related Experiment Video

Updated: Jan 3, 2026

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
09:27

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Published on: June 24, 2016

18.1K

A novel expression vector for Corynebacterium glutamicum with an auxotrophy complementation system.

Ying Li1, Yuqing Ai2, Junzheng Zhang3

  • 1College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, PR China.

Plasmid
|November 24, 2019
PubMed
Summary
This summary is machine-generated.

A new expression vector, pLY-4, was developed for Corynebacterium glutamicum. This vector enables efficient gene overexpression for industrial applications without needing antibiotics or IPTG, facilitating large-scale amino acid and vitamin production.

Keywords:
Auxotrophic complementation expressionConstitutive expression vectorCorynebacterium glutamicumL-methionine production

More Related Videos

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.6K
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

12.4K

Related Experiment Videos

Last Updated: Jan 3, 2026

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
09:27

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Published on: June 24, 2016

18.1K
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.6K
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

12.4K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Corynebacterium glutamicum is a key industrial microorganism for amino acid and vitamin production.
  • Existing gene overexpression tools in C. glutamicum often rely on inducible promoters (e.g., lacIq) and antibiotic resistance, limiting industrial scalability.
  • The need for IPTG and antibiotics in current vectors poses significant drawbacks for large-scale fermentation processes.

Purpose of the Study:

  • To construct and characterize a novel Escherichia coli-C. glutamicum shuttle expression vector, pLY-4.
  • To develop a vector suitable for industrial fermentation by eliminating the need for antibiotic selection and IPTG induction.
  • To demonstrate the utility of pLY-4 for heterologous gene expression and metabolic engineering in C. glutamicum.

Main Methods:

  • Construction of the pLY-4 shuttle vector from pXMJ19, incorporating a strong tacM promoter and a large multiple cloning site.
  • Integration of two selective markers: a chloramphenicol resistance gene (cat) for cloning and an alr gene for auxotrophy complementation.
  • Heterologous expression of the gapC gene in an auxotrophic C. glutamicum host using the pLY-4 vector.

Main Results:

  • The pLY-4 vector was successfully constructed, featuring a constitutive tacM promoter and dual selection markers.
  • The alr marker enabled stable vector replication in an auxotrophic host without antibiotic pressure, ideal for industrial fermentation.
  • Heterologous expression of gapC using pLY-4 facilitated L-methionine production, showcasing the vector's potential.

Conclusions:

  • The novel pLY-4 expression vector overcomes limitations of previous tools for C. glutamicum.
  • pLY-4 is suitable for industrial-scale fermentation due to its antibiotic-free selection mechanism.
  • This vector represents a valuable tool for C. glutamicum strain engineering and enhancing industrial bioproduction.