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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Frozen-Section Tissue Processing in Mohs Surgery.

Arif Aslam1, Sumaira Z Aasi2

  • 1Oxford Dermatology, Mount Hawthorn, Perth, Western Australia.

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [Et Al.]
|November 26, 2019
PubMed
Summary
This summary is machine-generated.

Mohs micrographic surgery (MMS) optimizes tissue preservation for skin cancer. This review details frozen-section processing techniques, offering practical tips to enhance accuracy and avoid margin errors in MMS procedures.

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Area of Science:

  • Dermatology
  • Surgical Pathology
  • Oncology

Background:

  • Mohs micrographic surgery (MMS) is a leading tissue-sparing method for skin cancer.
  • Variability exists in MMS mapping and tissue processing techniques among practitioners.

Purpose of the Study:

  • To review frozen-section tissue processing techniques specific to MMS.
  • To discuss variations in processing and provide optimization strategies.

Main Methods:

  • A literature search was conducted on PubMed focusing on MMS tissue processing.
  • Publications detailing tissue processing methods in MMS were analyzed.

Main Results:

  • Variations in debulking, embedding, and processing specific tissues (adipose, cartilage) were identified.
  • Techniques to prevent false-positive/negative margins and advances in immunohistochemistry are discussed.

Conclusions:

  • This article offers a practical guide to MMS tissue processing stages.
  • It provides techniques and pearls to improve accuracy and optimize practice for better patient outcomes.