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Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis

Tingting Chen1, Yong Wang2, Yang Yang1

  • 1Department of Medical Oncology, First Affiliated Hospital of Bengbu Medical College, Anhui Province Key Laboratory of Translational Cancer Research (Bengbu Medical College), 287 Changhuai Road, Bengbu, 233004, Anhui Province, People's Republic of China.

Biological Research
|November 27, 2019
PubMed
Abstract

Insights

Gramicidin exhibits anti-tumor effects against gastric cancer cells by inhibiting proliferation, migration, and inducing apoptosis. This peptide antibiotic shows potential for clinical application in treating gastric cancer.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Gastric cancer presents a significant global health challenge due to high morbidity and mortality.
  • Gramicidin, a peptide antibiotic, is primarily known for its antimicrobial properties.
  • The anti-cancer potential of gramicidin on gastric cancer remains largely unexplored.

Purpose of the Study:

  • To investigate the anti-cancer effects of gramicidin on human gastric cancer cells.
  • To elucidate the underlying mechanisms of gramicidin's action in gastric cancer.
  • To evaluate gramicidin's potential as a therapeutic agent for gastric cancer.

Main Methods:

  • Cell viability was assessed using CCK-8 assays.
  • Cell proliferation was evaluated through colony formation assays.
  • Cell migration, apoptosis, cell cycle, and protein expression (cyclinD1, Bcl-2, FoxO1 phosphorylation) were analyzed using wound healing, apoptosis/cell cycle analysis, and Western blot.

Main Results:

  • Gramicidin demonstrated significant toxicity to gastric cancer cells (SGC-7901, BGC-823) while sparing normal gastric mucosal cells (GES-1).
  • Gramicidin inhibited gastric cancer cell proliferation, migration, and induced apoptosis, with observed G2/M cell cycle arrest.
  • Down-regulation of cyclinD1, Bcl-2, and FoxO1 phosphorylation was observed following gramicidin treatment.

Conclusions:

  • Gramicidin exhibits significant anti-tumor activity against gastric cancer cells in vitro.
  • Gramicidin's mechanism involves inhibiting proliferation and migration, inducing apoptosis, and affecting key cell cycle and survival proteins.
  • These findings suggest gramicidin as a potential candidate for clinical application in gastric cancer treatment.
Keywords:
ApoptosisCell cycle arrestGramicidinProliferationSGC-7901

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