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A practical method for reducing the interference due to lipaemia in coagulation tests.

Chris Gardiner¹, Philip Lane¹, Hitesh Tailor²

¹Haemostasis Research Unit, University College London, London, UK.

International Journal of Laboratory Hematology

|November 27, 2019

Summary

This summary is machine-generated.

High-speed centrifugation effectively removes lipids from lipaemic plasma samples, enabling accurate coagulation testing. This method ensures reliable results for prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen, D-dimer, and von Willebrand factor (VWF) activity.

Keywords:

coagulation laboratory automation laboratory practice

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Area of Science:

Clinical Chemistry
Hematology
Laboratory Medicine

Background:

Lipaemic plasma samples pose challenges for optical coagulation analyzers.
High-speed centrifugation is a potential method to mitigate lipaemia.
The impact of centrifugation on haemostasis test results requires investigation.

Purpose of the Study:

To evaluate the effect of high-speed centrifugation on lipid reduction in lipaemic plasma.
To determine if centrifugation impacts key coagulation and haemostasis test results.
To assess the clinical significance of any observed changes in test parameters.

Main Methods:

Tested 26 lipaemic and 20 lipid-spiked plasma samples before and after centrifugation (10000 g for 10 minutes).
Assessed prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen, D-dimer, and von Willebrand factor (VWF) activity.
Utilized the Sysmex CS-5100 coagulation analyzer for all tests.

Main Results:

Centrifugation significantly reduced triglyceride and cholesterol levels, enabling clot detection in all samples.
No statistically significant differences were found in fibrinogen, D-dimer, or VWF activity post-centrifugation.
Minor, clinically insignificant changes were observed in PT and APTT after lipid removal.

Conclusions:

High-speed centrifugation effectively reduces lipaemia in plasma samples.
This method allows for reliable coagulation testing on optical analyzers.
Centrifugation does not introduce clinically significant alterations to PT, APTT, fibrinogen, D-dimer, or VWF activity measurements.