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Generation and Culturing of Primary Human Keratinocytes from Adult Skin
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Interleukin-38 interacts with destrin/actin-depolymerizing factor in human keratinocytes.

Dominique Talabot-Ayer1,2, Loïc Mermoud1,2, Julia Borowczyk1,3

  • 1Department of Pathology-Immunology, University of Geneva School of Medicine, Geneva, Switzerland.

Plos One
|November 27, 2019
PubMed
Summary
This summary is machine-generated.

Interleukin-38 (IL-38) is mainly found in the cytoplasm of human skin cells, not released outside. This cytokine interacts with destrin (DSTN), a protein involved in actin dynamics.

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Area of Science:

  • Immunology
  • Cell Biology
  • Dermatology

Background:

  • Interleukin-38 (IL-38), an IL-1 family cytokine, is expressed in skin keratinocytes and proposed to have anti-inflammatory roles.
  • Limited data exists on IL-38's biological functions and cellular behavior.

Purpose of the Study:

  • To determine the subcellular localization of IL-38 in human keratinocytes.
  • To identify IL-38 interacting partners within keratinocytes.

Main Methods:

  • Utilized IL-38 overexpressing keratinocytes (NHK/38) and a 3D reconstructed human epidermis (RHE) model.
  • Employed yeast two-hybrid screening, co-immunoprecipitation, and proximity ligation assays.
  • Examined IL-38 localization in cell monolayers, 3D models, and human skin sections.

Main Results:

  • IL-38 was primarily cell-associated and localized in the cytoplasm of keratinocytes, with minimal nuclear presence.
  • Destrin (DSTN) was identified as an IL-38 interacting partner.
  • IL-38 and DSTN showed partial co-localization in keratinocytes and were co-expressed in human epidermis.

Conclusions:

  • IL-38 predominantly resides in the cytoplasm of human keratinocytes and interacts with DSTN.
  • The functional significance of the IL-38/DSTN interaction in keratinocytes requires further investigation.