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Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors

Abraham Ochoa1, Frida Trejo1, Luis F Olguín2

  • 1Facultad de Química, Laboratorio de Biofísicoquímica, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, Mexico.

Methods in Molecular Biology (Clifton, N.J.)
|November 28, 2019
PubMed

Keywords:
Droplet microfluidicsDroplet-based assaysDrug discoveryEnzymatic assaysEnzyme inhibitionEnzyme kineticsHigh-throughput screeningMicrodropletsMicrofluidic deviceNatural products

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Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
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