Related Experiment Video

Updated: Aug 8, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors

Abraham Ochoa1, Frida Trejo1, Luis F Olguín2

  • 1Laboratorio de Biofísicoquímica. Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, México.

Methods in Molecular Biology (Clifton, N.J.)
|August 7, 2026
PubMed

Keywords:
AssaysDropletDroplet-Based assaysDrug discoveryEnzymatic assaysEnzyme inhibitionEnzyme kineticsFluorescenceHigh-throughput screeningLiquid chromatographyMicrodropletsMicrofluidic deviceMicrofluidicsNatural products

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Related Concept Videos

Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...