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Updated: Jan 3, 2026
Evaluation of the Storage Stability of Extracellular Vesicles
Published on: May 22, 2019
Freezing and storage effects on enzyme, electrolyte, and mineral compositions in canine prostatic fluid
Anne-Kathrin Umbach1,2, Klaus Failing3, Sandra Goericke-Pesch1,4
1Clinic for Obstetrics, Gynaecology and Andrology of Large and Small Animals with Veterinary Ambulance, Justus-Liebig-University, Giessen, Germany.
Background:
There is a paucity of information about the storage stability of seminal plasma components in literature.
Objectives:
This study investigates the potential effects of storage at -18.0°C on the enzyme activities and concentrations of selected electrolytes and minerals in canine prostatic fluid.
Methods:
Semen was obtained in fractions from ten male dogs, and the third ejaculate fraction was analyzed immediately after sampling and after storage at -18.0°C for 7 days. The following analytes were assessed: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), sodium, potassium, total calcium, inorganic phosphate, magnesium, zinc, and copper.
Results:
Whereas electrolyte and mineral concentrations were not affected by freezing and 7 days of storage, effects on the enzyme activities were variable. ALP, AST, and LDH activities were significantly decreased (P < .05) in the post-thaw samples compared with the freshly prepared samples.
Conclusions:
Because freezing and storage of seminal plasma at -18°C had an impact on the activity of some enzymes, analyses should be performed in fresh, not frozen samples.
