Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cellular compartmentation in ischemic myocardium: indirect analysis by electron probe.

L G Walsh1, J M Tormey

  • 1Department of Physiology, School of Medicine, University of California, Los Angeles 90024.

The American Journal of Physiology
|October 1, 1988
PubMed
Summary

Electron probe microanalysis revealed that myocardial sarcoplasmic reticulum (SR) calcium stores are stable during ischemia but significantly decrease upon reperfusion, indicating altered SR function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ARTIFACTUAL LOCALIZATION OF FERRITIN IN THE CILIARY EPITHELIUM IN VITRO.

The Journal of cell biology·2009
Same author

Potentiation of angiotensin II action by corticosteroids in vascular tissue.

Cardiovascular research·1996
Same author

Carbenoxolone damages endothelium and enhances vasoconstrictor action in aortic rings.

Hypertension (Dallas, Tex. : 1979)·1996
Same author

Corticosterone metabolism and effects on angiotensin II receptors in vascular smooth muscle.

Circulation research·1995
Same author

Potentiation of angiotensin II-stimulated vascular contraction by lithium.

The American journal of physiology·1995
Same author

Electron probe microanalysis of silicon and the role of the macrophage in proximal (capsule) and distant sites in augmentation mammaplasty patients.

Plastic and reconstructive surgery·1995

Area of Science:

  • Biochemistry
  • Cell Biology
  • Cardiovascular Science

Background:

  • Myocardial calcium handling is critical for cardiac function.
  • Ischemia-reperfusion injury significantly impacts cellular calcium balance.
  • The sarcoplasmic reticulum (SR) plays a key role in regulating intracellular calcium.

Purpose of the Study:

  • To quantify calcium distribution within myocardial cells using electron probe microanalysis (EPMA).
  • To investigate changes in subcellular calcium compartments, particularly the SR, during ischemia and reperfusion.
  • To elucidate the role of SR calcium content in myocardial response to ischemic events.

Main Methods:

  • Direct EPMA of myocardial cells, myofibrils, and mitochondria.
  • Indirect measurement of calcium within the sarcoplasmic reticulum (SR) compartment.

Related Experiment Videos

  • Calculation of SR calcium content by subtracting myofibril and mitochondrial data from total cell calcium.
  • Quantification of other elements in control, ischemic, and reperfused myocardium.

    • Main Results:

    • SR calcium content remained largely unchanged during global ischemia.
    • A marked depletion of SR calcium was observed after 5 minutes of reperfusion.
    • Other elemental compositions were also quantified across different conditions.

    Conclusions:

    • The sarcoplasmic reticulum (SR) is not significantly depleted of calcium during global ischemia.
    • Reperfusion triggers a significant loss of calcium from the SR.
    • These findings suggest that altered SR function contributes to myocardial dysfunction during reperfusion.