Related Experiment Video
Updated: Jan 3, 2026
Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
Published on: April 19, 2017
Monitoring Allogeneic CAR-T Cells Using Flow Cytometry
Agnieszka Jozwik1,2, Alan Dunlop1,3, Katy Sanchez1,3
1King's College London, London, UK.
Abstract:
Chimeric antigen receptor T cell (CAR-T) therapies have now entered mainstream clinical practice with two approved autologous CAR-T products targeting CD19 and numerous other products in early and late phase clinical trials. This has led to a demand for highly sensitive, specific, and easily reproducible methods to monitor CAR-T cells in patients. Here we describe a flow cytometry based protocol for detection of allogeneic CAR-T cells and for monitoring their phenotype and numbers in blood and bone marrow of patients following CAR-T treatment.
