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Updated: Jan 3, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
Late-stage difluoromethylation leading to a self-immobilizing fluorogenic probe for the visualization of enzyme
Jialing Jiang1, Qingwei Tan, Shuxuan Zhao
1State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. xiehexin@ecust.edu.cn.
Abstract:
Reported herein is a novel p-quinone methide-based self-immobilizing fluorogenic probe for the visualization of β-galactosidase activities in live cells. This easily prepared imaging reagent massively increases the fluorescence intensity and covalently links to the activation site with high efficiency upon enzymatic trigger.
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