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Plasma- and iron-regulated expression of high molecular weight outer membrane proteins by Pasteurella multocida
K P Snipes1, L M Hansen, D C Hirsh
1Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Abstract:
A strain of Pasteurella multocida of avian origin expressed high molecular weight outer membrane proteins when grown in turkey plasma or in brain-heart infusion broth containing the iron chelator dipyridyl. The proteins were not detected when this strain was grown in brain-heart infusion broth or in brain-heart infusion broth containing dipyridyl and excess iron.
Insights
Avian Pasteurella multocida outer membrane proteins were expressed during iron-limited growth conditions. Iron availability significantly impacts the expression of these high molecular weight proteins in this avian pathogen.
Area of Science:
- Microbiology
- Bacteriology
- Avian Pathogens
Background:
- Pasteurella multocida is an avian pathogen.
- Outer membrane proteins (OMPs) are crucial for bacterial-pathogen interactions.
- Iron availability is a key factor influencing bacterial gene expression and virulence.
Purpose of the Study:
- To investigate the expression of high molecular weight outer membrane proteins in an avian strain of Pasteurella multocida.
- To determine the effect of iron availability on the expression of these proteins.
Main Methods:
- Culturing an avian Pasteurella multocida strain under different iron conditions.
- Utilizing turkey plasma and brain-heart infusion (BHI) broth.
- Employing the iron chelator dipyridyl to induce iron-limited conditions.
- Supplementing with excess iron to counteract chelation.
Main Results:
- High molecular weight outer membrane proteins were detected when the bacteria were grown in turkey plasma or iron-limited BHI broth (containing dipyridyl).
- These specific proteins were not detected when the bacteria were grown in standard BHI broth or iron-replete BHI broth (containing dipyridyl and excess iron).
- Iron limitation appears to be a critical factor for the expression of these bacterial outer membrane proteins.
Conclusions:
- Iron availability significantly regulates the expression of specific high molecular weight outer membrane proteins in this avian Pasteurella multocida strain.
- These findings suggest a potential role for these iron-regulated proteins in the pathogenesis of avian pasteurellosis.
- Further research into these proteins could identify novel targets for vaccines or antimicrobials against avian Pasteurella multocida infections.