Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nano- and microplastics in the cardiovascular exposome: a new frontier in environmental risk.

European heart journal·2026

Same author

Air quality and health implications of exposure to ultrafine particle pollution.

Cardiovascular research·2026

Same author

Effects of the glucagon-like peptide-1 receptor agonist liraglutide on retinal endothelial function and oxidative stress during sepsis.

Frontiers in pharmacology·2026

Same author

Environmental risk factors and cardiovascular health.

European heart journal·2026

Same author

Individualized lesion-oriented test-bolus arterial-phase CT improves lesion enhancement and conspicuity in hepatocellular carcinoma.

European journal of radiology·2026

Same author

MyD88 in myeloid cells drives angiotensin II-induced vascular inflammation, is associated with prevalent heart failure, and predicts all-cause mortality in arterial hypertension.

European heart journal open·2026

Search research articles

Related Experiment Video

Updated: Jan 2, 2026

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

Published on: March 10, 2023

Apolipoprotein E Deficiency Causes Endothelial Dysfunction in the Mouse Retina.

Jenia Kouchek Zadeh¹, Mayagozel B Zhutdieva¹, Panagiotis Laspas¹

¹Department of Ophthalmology, University Medical Center, Johannes Gutenberg University Mainz, Langenbeckstr. 1, 55131 Mainz, Germany.

Oxidative Medicine and Cellular Longevity

|November 30, 2019

Summary

Chronic hypercholesterolemia in apolipoprotein E-deficient mice impairs retinal arteriole function and increases oxidative stress. However, antioxidant defenses prevent neuron and axon loss in the retina.

More Related Videos

Retinal Explant of the Adult Mouse Retina as an Ex Vivo Model for Studying Retinal Neurovascular Diseases

Retinal Explant of the Adult Mouse Retina as an Ex Vivo Model for Studying Retinal Neurovascular Diseases

Published on: December 9, 2022

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

Published on: September 26, 2018

Related Experiment Videos

Last Updated: Jan 2, 2026

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

Published on: March 10, 2023

Retinal Explant of the Adult Mouse Retina as an Ex Vivo Model for Studying Retinal Neurovascular Diseases

Retinal Explant of the Adult Mouse Retina as an Ex Vivo Model for Studying Retinal Neurovascular Diseases

Published on: December 9, 2022

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

Published on: September 26, 2018

Area of Science:

Ophthalmology
Cardiovascular Biology
Neuroscience

Background:

Atherogenic lipoproteins can cause tissue damage through impaired perfusion and oxidative stress.
Endothelial dysfunction and cell loss in the retina are potential consequences of chronic hypercholesterolemia.

Purpose of the Study:

To test the hypothesis that chronic hypercholesterolemia induces endothelial dysfunction and cell loss in the retina.
To investigate the role of apolipoprotein E deficiency in retinal vascular and neuronal health.

Main Methods:

Apolipoprotein E-deficient (ApoE-/-) mice and wild-type controls were used.
Retinal arteriole responses, oxidative and reactive nitrogen species, LOX-1 and NADPH oxidase expression, and redox gene expression were analyzed.
Retinal ganglion cell layer cell number and optic nerve axon number were quantified.

Main Results:

ApoE-/- mice exhibited impaired retinal arteriole responses to acetylcholine.
Increased expression of LOX-1 and NOX2, and elevated reactive oxygen species were observed in retinal blood vessels.
Despite increased oxidative stress markers and altered gene expression, retinal cell and axon numbers remained unchanged.

Conclusions:

Apolipoprotein E deficiency leads to endothelial dysfunction and oxidative stress in retinal arterioles, potentially causing retinal hypoxia.
Upregulation of antioxidant enzymes protects nonvascular retinal tissue from oxidative stress, preserving neurons and axons.