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Published on: June 16, 2021
Transcriptome datasets of gonadotropin-induced ESR2-regulated genes in rat oocytes
V Praveen Chakravarthi1, Subhra Ghosh1, Richita Roy1
1Department of Pathology and Laboratory Medicine, Kansas City, KS 66160, USA.
Abstract:
Disruption of estrogen receptor beta (ESR2) dysregulates oocyte maturation, which leads to failure of ovulation. We investigated ESR2-regulated genes during gonadotropin-induced oocyte maturation using RNA-sequencing. Through the administration of pregnant mare's serum gonadotropin (PMSG), synchronized follicle development was initiated in four-week-old wildtype and Esr2-null female rats. Forty-eight hours after the PMSG injection, human chorionic gonadotropin (hCG) was used for further maturation. Oocytes were collected from the ovaries 4 h after hCG injection. The total RNA was isolated from the oocytes and the whole oocyte transcriptome was determined by RNA-sequencing on the Illumina HiSeq4000 sequencer. RNA-sequencing data of wildtype and Esr2-null oocytes were analyzed, and differentially expressed genes were identified using the CLC Genomics Workbench. Whole oocyte transcriptome data of wildtype and Esr2-null oocytes were compared to identify the differentially expressed genes. Raw data are deposited to the NCBI Sequence Read Archive (SRA) and analyzed data are presented in this data article. These datasets can be utilized to identify the gonadotropin-induced genes in oocytes that are ESR2-regulated and important to oocyte maturation.
Insights
Estrogen receptor beta (ESR2) is crucial for ovulation. This study identified ESR2-regulated genes during gonadotropin-induced oocyte maturation, providing insights into female reproductive processes.
Area of Science:
- Reproductive Biology
- Genomics
- Endocrinology
Background:
- Estrogen receptor beta (ESR2) plays a critical role in regulating oocyte maturation and ovulation.
- Dysregulation of ESR2 can lead to ovulation failure, impacting female fertility.
Purpose of the Study:
- To investigate ESR2-regulated genes during gonadotropin-induced oocyte maturation using RNA-sequencing.
- To identify key genes influenced by ESR2 that are essential for successful oocyte maturation.
Main Methods:
- RNA-sequencing was performed on oocytes from wildtype and Esr2-null female rats.
- Gonadotropin stimulation (PMSG and hCG) was used to induce synchronized follicle development and oocyte maturation.
- Differential gene expression analysis was conducted using CLC Genomics Workbench to compare transcriptomes.
Main Results:
- Identified differentially expressed genes between wildtype and Esr2-null oocytes following gonadotropin stimulation.
- The RNA-sequencing data provides a comprehensive profile of the oocyte transcriptome regulated by ESR2.
- Raw and analyzed data are publicly available for further research.
Conclusions:
- ESR2 significantly influences the expression of specific genes during gonadotropin-induced oocyte maturation.
- The identified ESR2-regulated genes are important targets for understanding and potentially treating ovulation disorders.
- This dataset serves as a valuable resource for reproductive biology research.
