Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.5K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.5K
CRISPR01:59

CRISPR

57.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.3K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.6K
Homologous Recombination02:31

Homologous Recombination

62.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid large-scale morphological control of desulfurized fly ash enables high performance desulfurization material.

Waste management (New York, N.Y.)·2026
Same author

Stabilizing 1.93-eV ultrawide-bandgap perovskites for efficient triple-junction solar cells.

Nature communications·2026
Same author

Network meta-analysis of different preoperative hormone therapies for improving postoperative complications in pediatric hypospadias.

Frontiers in endocrinology·2026
Same author

Dual regulation of bile acids: ameliorating hepatic glycolipid disorders and restoring intestinal health in HFHC-challenged Yellow River carp (Cyprinus carpio L.).

BMC veterinary research·2026
Same author

Fabrication of hollow versus solid nanoparticles via co-assembly of soy protein isolate and gliadin: Structural characterization and functional properties.

Food research international (Ottawa, Ont.)·2026
Same author

High systolic blood pressure and stroke: evidence from the NHANES 1999-2023 and global burden of disease 2021.

Experimental biology and medicine (Maywood, N.J.)·2026

Related Experiment Video

Updated: Jan 2, 2026

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.0K

Dual-function chromogenic screening-based CRISPR/Cas9 genome editing system for actinomycetes.

Qiushui Wang1,2,3, Feng Xie1,3, Yaojun Tong4

  • 1Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

Applied Microbiology and Biotechnology
|December 3, 2019
PubMed
Summary

We developed an improved CRISPR/Cas9 genome editing system for actinobacteria, utilizing chromogenic reporters. This system accelerates the screening of edited mutants and plasmid curing, enhancing genome engineering efficiency.

Keywords:
ActinomycetesCRISPR/Cas9Chromogenic screeningPlasmid curinggusAidgS

More Related Videos

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.4K
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.6K

Related Experiment Videos

Last Updated: Jan 2, 2026

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.0K
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.4K
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.6K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Actinobacteria are crucial sources of valuable secondary metabolites.
  • Genome mining suggests underestimated secondary metabolite potential in actinobacteria.
  • CRISPR/Cas9 technology has revolutionized genetic manipulation in actinomycetes.

Purpose of the Study:

  • To develop a more efficient CRISPR/Cas9 system for actinobacteria genome engineering.
  • To streamline the screening of edited mutants and plasmid curing processes.
  • To demonstrate the system's applicability in both model and non-model actinomycetes.

Main Methods:

  • An updated CRISPR/Cas9 system was developed using GusA and IdgS chromogenic reporters.
  • The system was tested for gene and gene cluster deletion in Streptomyces coelicolor and Verrucosispora sp.
  • Efficiency was evaluated through positive clone screening and plasmid curing.

Main Results:

  • The updated CRISPR/Cas9 system significantly improved the speed and efficiency of genome editing.
  • Successful deletion of a single gene, a small gene cluster, and a large gene cluster was achieved.
  • The system facilitated rapid screening and efficient plasmid removal.

Conclusions:

  • The chromogenic reporter-based CRISPR/Cas9 system is a versatile tool for actinobacteria genome engineering.
  • This approach enhances efficiency and reduces labor in genetic manipulation of actinomycetes.
  • The system is broadly applicable across different actinobacterial species, including non-model organisms.