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Gene transcription in a temperature-sensitive mutant of RNA polymerase II
1Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140.
APMIS. Supplementum
|January 1, 1988
Abstract:
We have studied the transcription rates and the steady-state levels of cytoplasmic mRNA of selected genes in tsAF8 cells, a temperature-sensitive mutant of RNA polymerase II. At the restrictive temperature, the transcription rates decrease uniformly for all genes examined, while the corresponding decreases in steady-state levels of cytoplasmic mRNA vary for individual genes. tsAF8 cells can be advantageously used to study the half-line of mRNA without the use of perturbing drugs.