Effects of Fe₃O₄ nanoparticle fabrication and surface modification on Chlorella sp. harvesting efficiency

Harvesting is a critical step in microalgae-based biodiesel production. Oleaginous microalgae harvesting by magnetic nanomaterials has gained attention because of the advantages of higher efficiency, lower cost, and convenient operation. In the present study, Fe₃O₄ magnetic nanoparticles (MNPs) were fabricated using two different methods (chemical coprecipitation and thermal decomposition), modified with amino acid using three different approaches (ultrasonic, long-time mixing, and "one-step" approaches), and utilized for oleaginous microalgae Chlorella sp. HQ harvesting. The results showed that the Fe₃O₄ MNPs synthesized by the chemical coprecipitation method achieved superior performance when considering both harvesting efficiency and fabrication cost. For the amino-acid modification, the one-step approach outcompeted the other approaches. At a dosage of 200 mg/L, the optimized Fe₃O₄@Arginine MNPs could achieve a harvesting efficiency of 95% with a low cost of only 347 USD/t of harvested algal biomass. Both the amino-acid content on the NPs and the number of amino groups in the amino acid molecules played a role in improving the harvesting performance.