Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering Organelle-Gated Reporters for Imaging Subcellular Enzyme Activity in Living Cells.

Journal of the American Chemical Society·2026
Same author

A general strategy for high-efficiency live bacteria imaging and targeted phototherapy.

Chemical science·2026
Same author

Porous Glycine-Modified LaFeO<sub>3</sub> for Highly Selective Ethanol Sensing: Band Gap Engineering Driven by Organic-Inorganic Hybridization.

ACS sensors·2026
Same author

The nigrostriatal dopaminergic pathway mediates state-dependent emergence from propofol anesthesia in mice.

Brain research bulletin·2026
Same author

Subthalamic CaMKIIα-expressing neurons facilitate recovery from propofol anesthesia via the STN-ventral pallidum pathway.

Cell biology and toxicology·2026
Same author

Insomnia Pathogenesis and Multidimensional Mechanisms of Acupuncture: A Narrative Review.

International journal of general medicine·2026

Related Experiment Video

Updated: Jan 2, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.0K

A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy.

Lu Wang1, Mai Tran2, Elisa D'Este3

  • 1Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany. Lu.Wang@mr.mpg.de.

Nature Chemistry
|December 4, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to create fluorogenic probes for live-cell nanoscopy. These probes offer improved cell permeability and reduced background signals, enabling clearer visualization of cellular processes.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
08:14

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Published on: November 13, 2015

11.3K

Related Experiment Videos

Last Updated: Jan 2, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.0K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
08:14

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Published on: November 13, 2015

11.3K

Area of Science:

  • Cellular Biology
  • Biophysics
  • Chemical Biology

Background:

  • Live-cell fluorescence nanoscopy requires effective fluorescent probes for molecular-scale cellular studies.
  • Existing probes often exhibit poor cell permeability and high background noise, limiting their utility.
  • A need exists for advanced probes that overcome these limitations for enhanced cellular imaging.

Purpose of the Study:

  • To develop a general strategy for transforming conventional fluorophores into high-performance fluorogenic probes.
  • To enhance probe cell permeability and minimize unspecific background signals for live-cell nanoscopy.
  • To create versatile, multicolour fluorogenic probes for various cellular targets and applications.

Main Methods:

  • A chemical modification strategy was employed, converting a carboxyl group in rhodamine-based fluorophores to an electron-deficient amide.
  • This modification rationally tunes the equilibrium between a fluorescent zwitterionic form and a non-fluorescent, cell-permeable spirolactam form.
  • The equilibrium shift towards the fluorescent form upon cellular target binding was investigated.

Main Results:

  • The developed strategy successfully transformed regular fluorophores into fluorogenic probes with superior cell permeability and low background.
  • A significant fluorescence increase, up to 1,000-fold, was observed upon probe binding to cellular targets.
  • Various colour fluorogenic probes were synthesized for different cellular targets, demonstrating wash-free, multicolour live-cell nanoscopy capabilities.

Conclusions:

  • A novel and generalizable method for creating advanced fluorogenic probes has been established.
  • These probes significantly improve the quality and scope of live-cell nanoscopy.
  • The developed probes offer a powerful tool for detailed molecular-level investigations in cellular biology.