Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

3.2K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
3.2K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.9K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.9K
Surface Membrane Barriers01:18

Surface Membrane Barriers

2.5K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
2.5K
Cells of the Epidermis01:24

Cells of the Epidermis

6.6K
The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
6.6K
Layers of the Epidermis01:21

Layers of the Epidermis

7.5K
The epidermis, the outermost layer of the skin, is composed of several distinct layers. From deep to superficial, the layers of the epidermis are as follows:
Stratum Basale
Stratum basale, also known as the stratum germinativum, is the deepest layer of the epidermis. It is composed of a single layer of actively dividing cells called basal cells or basal keratinocytes. These cells constantly undergo cell division to replenish the upper layers of the epidermis. Additionally, melanocytes, which...
7.5K
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

7.0K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Fidelity Modeling of Laser Levels via Pulse-Window Software Lock-In PSD Sensing.

Sensors (Basel, Switzerland)·2026
Same author

Single-cell transcriptomic mendelian randomization and co-localization reveal immune-mediated regulatory mechanisms and drug targets in atopic dermatitis.

International immunopharmacology·2026
Same author

Cardiac resident macrophages: the emerging role in arrhythmogenesis.

Frontiers in immunology·2026
Same author

Erratum to "Deep insights and clinical benefits from the comprehensive cohort of fetal skeletal dysplasia in China"[Journal of Genetics and Genomics 52 (2025) 1524-1536].

Journal of genetics and genomics = Yi chuan xue bao·2026
Same author

Role of LIGHT in the Inflammatory Mechanisms of Psoriasis via Upregulation of Proliferation and Cytokine Production of Keratinocytes.

Immunity, inflammation and disease·2026
Same author

Epidemiological characteristics in Shanghai and a prognostic nomogram for 90 day adverse outcomes in acute hepatitis E.

Hepatology international·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jan 2, 2026

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
08:45

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

5.8K

Dermal-Epidermal Separation by Chemical.

Liyan Jian1, Yu Cao1, Ying Zou2

  • 1Institute of Precision Medicine, The Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2019
PubMed
Summary
This summary is machine-generated.

Researchers describe a chemical method for separating skin layers. This technique is crucial for pharmacological, toxicological, and biological investigations, offering a valuable tool for skin research.

Keywords:
ChemicalDermisEpidermisSeparationSodium thiocyanate

More Related Videos

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
07:55

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles

Published on: February 7, 2019

11.4K
Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
09:14

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

16.7K

Related Experiment Videos

Last Updated: Jan 2, 2026

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
08:45

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

5.8K
Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
07:55

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles

Published on: February 7, 2019

11.4K
Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
09:14

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

16.7K

Area of Science:

  • Dermatology
  • Pharmacology
  • Toxicology
  • Biology

Background:

  • The skin comprises three distinct layers: epidermis, dermis, and hypodermis.
  • Dermal-epidermal separation is a fundamental technique in biological, pharmacological, and toxicological research.
  • Existing separation methods include chemical, enzymatic, and thermal approaches, each with unique benefits and drawbacks.

Purpose of the Study:

  • To detail a specific chemical method for achieving dermal-epidermal separation.
  • To provide a reproducible protocol for researchers investigating skin structure and function.

Main Methods:

  • The study focuses on a chemical-based approach for separating the epidermis from the dermis.
  • Detailed procedural steps for the chemical separation technique are presented.

Main Results:

  • The described chemical method effectively separates epidermal components from the dermis.
  • This technique provides a viable option for researchers requiring precise dermal-epidermal separation.

Conclusions:

  • Chemical separation of the skin's epidermal and dermal layers is an effective technique.
  • The choice of separation method should be tailored to specific research objectives in pharmacology, toxicology, and biology.