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Ex-vivo perfusion of surgically removed organs
12nd Department of Surgery, Hamamatsu University School of Med., Japan.
Abstract:
Surgically removed organs were perfused with oxygenated perfluorochemical blood substitute(FDA-O2). The perfusion was carried out using resected mouse liver. ATP level of perfused liver was remaining normal until 80 min. ATP peak disappeared when perfusion was discontinued, and it reappeared on reperfusion. No significant ischemic changes were demonstrated. Ex-vivo autoradiogram successfully labelled the S phase cells in various organs and various diseases. The absorptive study is also performed. Ex-vivo organ perfusion with FDA-O2 is able to keep the resected human organs viable, and is valuable new method to analyze the function and histogenesis of pathology.
Insights
Ex-vivo organ perfusion using FDA-O2, an oxygenated blood substitute, maintained mouse liver viability and ATP levels. This technique shows promise for preserving resected human organs and studying disease pathology.
Area of Science:
- Biomedical Engineering
- Organ Perfusion Technology
- Pathology Research
Background:
- Maintaining organ viability after surgical resection is critical for transplantation and research.
- Current methods for preserving resected organs have limitations in sustaining function and preventing ischemic damage.
Purpose of the Study:
- To evaluate the efficacy of ex-vivo organ perfusion with an oxygenated perfluorochemical blood substitute (FDA-O2) in maintaining organ viability.
- To assess the potential of this method for analyzing organ function and the histogenesis of pathology.
Main Methods:
- Surgically resected mouse livers were perfused with oxygenated FDA-O2.
- Adenosine triphosphate (ATP) levels were monitored during perfusion and reperfusion.
- Ex-vivo autoradiography was employed to label S phase cells.
- Absorptive studies were conducted.
Main Results:
- Perfusion with FDA-O2 maintained normal ATP levels in mouse liver for up to 80 minutes.
- ATP levels decreased upon perfusion cessation and recovered upon reperfusion, indicating preserved viability.
- No significant ischemic changes were observed in the perfused organs.
- Ex-vivo autoradiography successfully labeled S phase cells, demonstrating the utility for cellular analysis.
Conclusions:
- Ex-vivo organ perfusion with FDA-O2 effectively preserves the viability of resected organs, including human organs.
- This technique offers a valuable new method for analyzing organ function and understanding the histogenesis of various pathologies.