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Updated: Jan 2, 2026

A Low Cost Setup for Behavioral Audiometry in Rodents
Published on: October 16, 2012
Temporary hearing threshold shift in harbor seals (Phoca vitulina) due to a one-sixth-octave noise band centered at
Ronald A Kastelein1, Lean Helder-Hoek1, Suzanne Cornelisse1
1Sea Mammal Research Company (SEAMARCO), Julianalaan 46, 3843 CC Harderwijk, the Netherlands.
Abstract:
Temporary hearing threshold shifts (TTSs) were investigated in two adult female harbor seals after exposure for 60 min to a continuous one-sixth-octave noise band centered at 16 kHz (the fatiguing sound) at sound pressure levels of 128-149 dB re 1 μPa, resulting in sound exposure levels (SELs) of 164-185 dB re 1 μPa2s. TTSs were quantified at the center frequency of the fatiguing sound (16 kHz) and at half an octave above that frequency (22.4 kHz) by means of a psychoacoustic hearing test method. Susceptibility to TTS was similar in both animals when measured 8-12 and 12-16 min after cessation of the fatiguing sound. TTS increased with increasing SEL at both frequencies, but above an SEL of 174 dB re 1 μPa2s, TTS was greater at 22.4 kHz than at 16 kHz for the same SELs. Recovery was rapid: the greatest TTS, measured at 22.4 kHz 1-4 min after cessation of the sound, was 17 dB, but dropped to 3 dB in 1 h, and hearing recovered fully within 2 h. The affected hearing frequency should be considered when estimating ecological impacts of anthropogenic sound on seals. Between 2.5 and 16 kHz the species appears equally susceptible to TTS.
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