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Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs
Published on: June 20, 2016
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Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana Using TAL Effector Nucleases
1Organelle Biology, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam, Germany. forner@mpimp-golm.mpg.de.
Methods in Molecular Biology (Clifton, N.J.)
|December 5, 2019
Abstract:
TAL effector nucleases (TALENs) are powerful tools to create specific knockout mutants in plants. The use of an optimized TALEN backbone and the choice of promoters that are strongly active in the stem cells of the shoot apical meristem are key to a high rate of heritable targeted mutations. Recommendations for construct design and screening for mutants are given in this chapter.
Keywords:
Arabidopsis thalianaGenome editingGermlineHeritableKnockoutNHEJStem cell-specific promoterTALENMore Related Videos
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