Related Experiment Video
Updated: Jan 2, 2026
Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data
Published on: April 26, 2016
3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox
Viktoriya V Lavrekha1,2, Taras Pasternak3, Klaus Palme3
1Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
Abstract:
The protocol allows to define and characterize mitosis distribution patterns in the plant root meristem. The method does not require genetic markers, which makes it applicable to plants of different non-transgenic genotypes, including ecotypes, mutants, and non-model plant species. Computer analysis of the mitosis distribution in three dimensions with iRoCS Toolbox identifies statistically significant changes in proliferation activity within specific root tissues and cell lineages.
