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Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma

Zuojun Zhang1,2, Ming Zhao1, Guojie Wang1

  • 1Upper Limb Injury Treatment Center, Luoyang Orthopedic Hospital of Henan Province, Luoyang, China.

Cell & Bioscience
|December 5, 2019
PubMed
Abstract

Insights

Circular RNA circ_0051079 acts as an oncogene in osteosarcoma by regulating miR-26a-5p and TGF-β1. This finding suggests circ_0051079 could be a potential biomarker for osteosarcoma diagnosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma is a prevalent bone cancer affecting children and adolescents.
  • Circular RNAs (circRNAs) are implicated in cancer development by interacting with microRNAs (miRNAs).
  • The specific role of circRNAs in osteosarcoma remains largely uncharacterized.

Purpose of the Study:

  • To investigate the role and molecular mechanism of circRNAs in osteosarcoma.
  • To identify specific circRNAs involved in osteosarcoma progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess circRNA expression.
  • Bioinformatic analysis to predict circRNA-miRNA interactions.
  • Luciferase activity assays to validate molecular interactions.
  • Cellular function assays to evaluate the impact on proliferation and metastasis.

Main Results:

  • Circ_0051079 expression was significantly elevated in osteosarcoma tissues and cells.
  • Circ_0051079 functions as a sponge for miR-26a-5p.
  • TGF-β1 was identified as a target mRNA of miR-26a-5p.
  • The circ_0051079/miR-26a-5p/TGF-β1 axis was shown to regulate osteosarcoma cell proliferation and metastasis.

Conclusions:

  • Circ_0051079 acts as an oncogene in osteosarcoma.
  • The circ_0051079/miR-26a-5p/TGF-β1 pathway plays a crucial role in osteosarcoma progression.
  • Circ_0051079 holds potential as a diagnostic biomarker for osteosarcoma.
Keywords:
Hsa_circ_0051079OsteosarcomaTGF-β1miR-26a-5p

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