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Circular RNA circ-SMAD7 promoted glioma cell proliferation and metastasis by upregulating PCNA

C-Y Zuo1, W Qian, C-J Huang

  • 1Department of Neurosurgery, The Ninth People's Hospital of Suzhou, Suzhou, China. zuochangyang@163.com.

European Review for Medical and Pharmacological Sciences
|December 5, 2019
PubMed
Abstract

Insights

Circular RNA SMAD7 (circ-SMAD7) promotes glioma progression by upregulating proliferating cell nuclear antigen (PCNA). Targeting the circ-SMAD7/PCNA pathway may offer a new therapeutic strategy for glioma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are a class of regulatory RNAs implicated in tumor progression.
  • Dysregulation of circRNAs is observed in various cancers, including glioma.
  • The specific role of circ-SMAD7 in glioma pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the function of circ-SMAD7 in glioma progression.
  • To explore the underlying molecular mechanism of circ-SMAD7 in glioma.
  • To evaluate circ-SMAD7 as a potential therapeutic target for glioma.

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to detect circ-SMAD7 expression in glioma tissues.
  • Cellular assays including proliferation, cell cycle, migration, and invasion assays to assess circ-SMAD7 function.
  • Western blot and qRT-PCR to analyze the interaction between circ-SMAD7 and proliferating cell nuclear antigen (PCNA).

Main Results:

  • Circ-SMAD7 expression was significantly higher in glioma tissues compared to adjacent samples.
  • Downregulation of circ-SMAD7 inhibited glioma cell proliferation, migration, and invasion, and regulated the cell cycle.
  • Circ-SMAD7 positively correlated with PCNA expression, and its knockdown reduced PCNA mRNA and protein levels.

Conclusions:

  • Circ-SMAD7 promotes glioma proliferation and metastasis by upregulating PCNA.
  • The circ-SMAD7/PCNA axis represents a potential novel therapeutic strategy for glioma treatment.

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