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Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
Published on: November 10, 2023
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Global Transcriptomic Profiling of the Bone Marrow Stromal Microenvironment during Postnatal Development, Aging, and
Patrick M Helbling1, Elena Piñeiro-Yáñez2, Rahel Gerosa1
1Department of Medical Oncology and Hematology, University Hospital and University of Zurich, 8091 Zurich, Switzerland.
Cell Reports
|December 5, 2019
Summary
Bone marrow stromal cells change transcriptionally throughout life. Aging bone marrow stroma exhibits increased inflammation, impacting hematopoietic stem cell niches.
Area of Science:
- Hematology
- Developmental Biology
- Immunology
Background:
- Bone marrow (BM) stromal cells regulate hematopoiesis and form stem cell niches.
- Transcriptional changes in stromal cells across lifespan and stress are poorly understood.
Purpose of the Study:
- To analyze transcriptional alterations in key BM stromal subsets during aging.
- To investigate how aging impacts stromal cell function and hematopoietic regulation.
Main Methods:
- RNA sequencing of four principal stromal subsets (reticular, PDGFR-α+, sinusoidal, arterial endothelial cells) from mice at different life stages (postnatal, adult, aged).
Main Results:
- Defined molecular signatures for distinct stromal subsets.
- Identified significant transcriptional reprogramming from juvenile to adult stages, affecting hematopoietic, immune, and matrix-related genes.
- Observed progressive upregulation of pro-inflammatory gene expression in aged stroma.
Conclusions:
- Aging BM stroma shows increased inflammatory gene expression, mirroring pathways induced by microbial stimuli.
- These age-related inflammatory changes likely underpin adaptations in BM hematopoietic function.
Keywords:
agingbone marrow microenvironmenthematopoietic stem cellsinflammationnichesstromal cellstranscriptomicsMore Related Videos
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