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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

Systematic Y2H Screening Reveals Extensive Effector-Complex Formation.

André Alcântara¹, Jason Bosch¹, Fahimeh Nazari^1,2

¹Gregor Mendel Institute of Molecular Plant Biology, Vienna, Austria.

Frontiers in Plant Science

|December 6, 2019

Summary

This summary is machine-generated.

Pathogenic effectors from Ustilago maydis, a fungus infecting maize, can interact with each other. This discovery of effector complex formation adds complexity to understanding plant-pathogen molecular arms races and effector evolution.

Keywords:

Ustilago maydis effector proteins plant pathogen protein–protein interaction network yeast-two-hybrid

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Area of Science:

Plant-pathogen interactions
Molecular plant pathology
Fungal effector biology

Background:

Pathogens secrete effectors to manipulate host interactions during infection.
Plants and pathogens engage in a molecular arms race, driving rapid co-evolution.
While plant immune receptor networks are known, pathogen effector interactions are poorly understood.

Purpose of the Study:

To investigate if Ustilago maydis effector candidates interact with each other.
To explore the potential role of effector complex formation in plant immunity manipulation.
To add a new layer of complexity to effector research and evolution studies.

Main Methods:

Systematic yeast-two-hybrid screening was employed.
A preliminary pooled screen identified candidates.
63 putative effectors were tested in one-on-one interactions against a library of ~300 candidates.

Main Results:

126 out of ~300 effector candidates showed self-interaction or interaction with other effectors.
A significant number of Ustilago maydis effectors form complexes.
The functional relevance of these interactions requires further investigation.

Conclusions:

Pathogenic effectors can form complexes with themselves and other effectors.
Effector complex formation represents a novel aspect of plant-pathogen interactions.
Considering effector interactomes is crucial for understanding effector evolution and function.