Effects o;f vitrectomy infusion solutions containing oxacillin, methicillin, or lincomycin
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Abstract:
Experiments conducted in rabbits showed no toxic effects on the retina by electroretinogram after vitrectomy when the infusion fluid contained as much as 50 microgram/ml of methicillin or lincomycin or 20 microgram/ml oxacillin. An abnormal electroretinogram was found in eyes receiving oxacillin in a concentration of 50 microgram/ml. Histologic studies showed ocular damage with concentrations of 50 microgram/ml of methicillin or lincomycin or 20 microgram/ml of oxacillin. All antibiotics were nontoxic to retina at 10 microgram/ml concentration, both by ERG and histology.
Insights
Antibiotics methicillin, lincomycin, and oxacillin showed no retinal toxicity at 10 µg/ml during vitrectomy in rabbits. Higher concentrations caused ocular damage and abnormal electroretinograms (ERG), indicating potential risks.
Area of Science:
- Ophthalmology
- Pharmacology
- Toxicology
Background:
- Intraocular antibiotics are used during vitrectomy to prevent infection.
- Determining safe antibiotic concentrations for ocular tissues is crucial.
Purpose of the Study:
- To evaluate the retinal toxicity of methicillin, lincomycin, and oxacillin during vitrectomy in a rabbit model.
Main Methods:
- Rabbits underwent vitrectomy with infusion fluids containing varying concentrations of antibiotics.
- Retinal toxicity was assessed using electroretinography (ERG) and histologic examination.
Main Results:
- No toxic effects were observed at 10 µg/ml for any antibiotic.
- Ocular damage and abnormal ERGs occurred at higher concentrations (50 µg/ml for methicillin/lincomycin, 20 µg/ml for oxacillin).
Conclusions:
- Safe intraocular concentrations for these antibiotics during vitrectomy appear to be below 10 µg/ml.
- Higher concentrations pose a risk of retinal toxicity and ocular damage.