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Hot EVs - How temperature affects extracellular vesicles.

Eilien Schulz1, Anna Karagianni2, Marcus Koch3

  • 1Biogenic Nanotherapeutics Group (BION), Helmholtz Centre for Infection Research (HZI), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Campus E8.1, Saarbrücken 66123, Germany; Department of Pharmacy, Saarland University, Campus E8.1, Saarbrücken 66123, Germany.

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V
|December 6, 2019
PubMed
Summary
This summary is machine-generated.

Extracellular vesicles (EVs) and outer membrane vesicles (OMVs) show remarkable heat stability. These biological nanoparticles maintain their integrity and function even after exposure to high temperatures, crucial for therapeutic applications.

Keywords:
AutoclavingDrug carriersExtracellular vesiclesFlow cytometryHeat stabilityLymphoblastoid cellsMyxobacteriaOuter membrane vesicles

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Area of Science:

  • Biotechnology
  • Nanomedicine
  • Cell Biology

Background:

  • Extracellular vesicles (EVs) and outer membrane vesicles (OMVs) are crucial for biological processes and hold therapeutic potential.
  • Understanding the stability of EVs and OMVs is vital for their clinical application and formulation development.
  • Limited data exists on the heat stability of EVs and OMVs.

Purpose of the Study:

  • To investigate the heat stability of mammalian EVs and bacterial OMVs.
  • To assess the impact of varying temperatures and durations on vesicle characteristics and functionality.
  • To provide insights into vesicle stability for bioengineering and therapeutic formulation.

Main Methods:

  • B lymphoblastoid cell-derived EVs and myxobacterial Sorangiineae-derived OMVs were used as model systems.
  • Vesicles were subjected to temperatures ranging from 37°C to 100°C for different durations and autoclaved.
  • Physico-chemical analyses (size, particle/protein concentration) and flow cytometry were performed.

Main Results:

  • Minor alterations in size, particle concentration, and protein concentration were observed, especially at 37°C.
  • Flow cytometry indicated that heat-treated EVs and OMVs retained their ability to be taken up by macrophage-like cells.
  • Both mammalian and bacterial vesicles demonstrated intrinsic stability at physiological temperatures.

Conclusions:

  • Mammalian and bacterial vesicles exhibit significant intrinsic heat stability.
  • These findings support the potential of EVs and OMVs in therapeutic applications requiring thermal stability.
  • The study provides essential data for vesicle formulation and bioengineering strategies.