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Protection against complement-mediated cell damage by Ca2+ and Zn2+
K J Micklem1, G M Alder, C D Buckley
1Nuffield Department of Pathology, John Radcliffe Hospital, Oxford, UK.
Complement (Basel, Switzerland)
|January 1, 1988
Abstract:
Ca2+ and Zn2+ prevent antibody-dependent complement-induced permeability changes in tonsil lymphocytes and Lettre cells. Lactate dehydrogenase leaks out from Lettre cells at high complement:cell ratios, under which conditions higher concentrations of Ca2+ and Zn2+ are required for protection. Ca2+ and Zn2+ do not inhibit complement activation or C9 binding to Lettre cells, and prevent leakage through preformed lesions. It is concluded that the extent of complement-induced membrane damage depends on the concentration of extracellular Ca2+, and may be modulated by changes in extracellular Ca2+ or Zn2+.