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Related Experiment Videos

An EPROM-based programmable contour generator for use in flow cytometry.

D M Wheeless1, J L Cambier, L L Wheeless

  • 1Department of Pathology, University of Rochester Medical Center, New York 14642.

Cytometry
|September 1, 1988
PubMed
Summary
This summary is machine-generated.

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A new erasable programmable read-only memory (EPROM) contour generator creates artificial flow cytometry data. This tool aids in developing and testing slit-scan instrumentation and analysis algorithms without needing actual specimens.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Instrumentation

Background:

  • Flow cytometry generates contour plots representing cellular or object characteristics.
  • Developing and testing flow cytometry instrumentation, particularly slit-scan systems, requires reliable and reproducible data.
  • Current methods may necessitate actual specimens or full instrument operation for testing.

Purpose of the Study:

  • To develop a novel contour generator for flow cytometry applications.
  • To provide a tool for testing slit-scan instrumentation and analysis algorithms.
  • To enable the generation of artificial, reproducible contour data.

Main Methods:

  • Fabrication of an erasable programmable read-only memory (EPROM) based contour generator.

Related Experiment Videos

  • The generator produces analog waveforms mimicking fluorescence or light-scatter intensity distributions.
  • Programmable control over contour parameters: height, width, offset, and rate.

    • Main Results:

    • The EPROM contour generator successfully produces artificial contour waveforms.
    • Generated contours can be customized for specific test applications or to simulate real events.
    • The generator operates independently of specimens and full flow instrument functionality.

    Conclusions:

    • The EPROM contour generator offers a valuable tool for flow cytometry research and development.
    • It facilitates the testing of slit-scan instrumentation and analysis algorithms in a controlled, reproducible manner.
    • The device is particularly useful for applications requiring consistent, predetermined contour characteristics.