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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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mir-21-5p inhibits the progression of human chondrosarcoma by regulating CCR7/STAT3/NF-κB pathway

Guosong Li1, Yanjun Yang1, Siliang Xu1

  • 1Department of Orthopedics, Longgang Orthopedics Hospital of Shenzhen, Shenzhen, Guangdong, China.

Connective Tissue Research
|December 10, 2019
PubMed

Insights

MicroRNA-21-5p (miR-21-5p) suppresses chondrosarcoma (CS) progression by inhibiting C-C chemokine receptor type 7 (CCR7) expression. This mechanism reduces cell proliferation, migration, and epithelial-mesenchymal transition, offering a potential therapeutic target for CS.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are crucial in chondrosarcoma (CS) development.
  • The specific role and mechanism of miR-21-5p in CS progression are not fully understood.

Purpose of the Study:

  • To investigate the role of miR-21-5p in chondrosarcoma (CS) progression.
  • To elucidate the underlying molecular mechanisms of miR-21-5p in CS.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for miR-21-5p expression.
  • Cell counting kit-8 (CCK-8) and Transwell assays for cell behavior.
  • Dual-luciferase reporter assay, Western blot, and xenograft models.

Main Results:

  • miR-21-5p was downregulated in CS tissues and correlated with poor survival.
  • miR-21-5p inhibited cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in vitro and in vivo.
  • miR-21-5p targets C-C chemokine receptor 7 (CCR7) mRNA, inhibiting STAT3 and NF-κB signaling pathways.

Conclusions:

  • miR-21-5p acts as a tumor suppressor in chondrosarcoma (CS).
  • Targeting CCR7 by miR-21-5p inhibits CS cell proliferation, invasion, and EMT via STAT3 and NF-κB pathways.
  • miR-21-5p represents a potential therapeutic target for chondrosarcoma (CS).
Keywords:
CCR7CSNF-κBSTAT3miR-21-5p

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